YK052 Mouse Leptin ELISA Kit
Leptin, which is a product of ob gene, is a protein consisting of 146 amino acids and it is secreted from white adipose tissue. It is known that leptin acts on hypothalamus to decrease food intake and reduce body weight, body fat, blood sugar and blood insulin in a healthy and an ob/ob mice. Further, gene expression of neuropeptide Y (NPY) is suppressed by this protein. Recently, radioimmunoassay for leptin determination in human plasma has become available and leptin level in human patient group with obesity was found to increase in comparison with that in normal group. The plasma level well correlated with body fat and these observations showed clearly that leptin concentration in human plasma reflects tissue fat weight. The measurement of leptin in plasma or serum may be a good index of obesity. Although mouse leptin shows a high homology (96%) with rat leptin in amino acid sequences, it is observed that substitution of quite a number of residues occurs between human and mouse leptin. These findings have urged us to develop highly sensitive immunoassay system specific to rat or mouse leptin.
Yanaihara Institute Inc. already has rat leptin ELISA kit (YK050 and YK051), and now we have developed mouse leptin ELISA kit for measuring mouse leptin in its serum. It will be a specifically useful tool for leptin researches.
▼The assay kit can measure mouse leptin within the range of 0.313-20 ng/mL.
▼ The assay is completed within 6.5 hr.
▼ With one assay kit, 40 samples can be measured in duplicate.
▼Test sample: Mouse serum
▼ Sample volume: 25 μL
▼ The 96-wells plate in kit is consisted by 8-wells strips, and the strips can be used separately.
▼ Precision and reproducibility
Intra-assay CV（％） Mouse serum 5.01-9.84
Inter-assay CV（％） Mouse serum 4.37-7.71
< Specificity >
This ELISA kit is highly specific to mouse leptin. It shows 33.8 % crossreactivity to rat leptin and no crossreactivity to human leptin.