YK081 Mouse/Rat PYY EIA
This enzyme immunoassay (EIA) kit is a stable and convenient assay system for peptide YY (PYY). PYY was isolated initially by Tatemoto et al. (1980 ) from the extract of pig duodenum and shown to be a polypeptide consisting of 36 amino acid residues. PYY is homologous to pancreatic polypeptide (PP) and neuropeptide Y (NPY). PYY is localized mainly in endocrine cells in the intestine (ileum, colon, and rectum) showing an inhibitory action on contraction of the gastrointestinal tract and on secretion of pancreatic and gastric juice. PYY is released by taking diet. The PYY level decreases after resection of the intestine possibly are due to the decrease in number of the endocrine cells secreting PYY. The EIA kit is prepared by using synthetic mouse/rat PYY (3-36) as standard antigen and biotinylated mouse/rat PYY (3-36) as labeled antigen. The kit contains specific polyclonal antibody recognized to the amino acid sequence of Mouse/rat PYY.
The mouse/rat PYY sequence: Y-P-A-K-P-E-A-P-G-E-D-A-S-P-E-E-L-S-R-Y-Y-A-S-L-R-H-Y-L-N-L-V-T-R-Q-R-Y-NH2
▼ The assay kit can measure mouse/rat PYY within the range of 0.15-12.5 ng/mL.
▼ The assay completes within 18.5h. + 1.5 h.
▼ With one assay kit, 42 samples can be measured in duplicate.
▼ Test sample: mouse/rat plasma or serum.
▼ Sample volume: 25 μL.
▼ The 96-well plate of this kit is consists of 8-well strips, The divided use by strips is possible at user’s option.
▼ Precision and reproducibility
Intra-assay CV (%) 3.1～9.8,
Inter-assay CV (%) 4.2～14.2
< Specificity>
The EIA kit shows 100% cross reactivity to mouse/rat PYY (3-36) and 115% to mouse/rat PYY (1-36). Cross reactivity was not observed in the assay range with mouse/rat NPY that has similar amino acid sequence with mouse/rat PYY. No cross reactivity with GLP-1 (7-36)-NH2, GLP-1 (1-37), and rat GLP-2 were observed.