YK140 Rat GLP-2 EIA Kit
The proglucagon gene is expressed in both pancreatic A cell and intestinal L cell. Tissue-specific posttranslational processing of proglucagon by the prohormone convertase produced the different proglucagon derived peptides (PGDPs) in both pancreas and intestine. The most notable pancreatic PGDP is glucagon, whereas the L cell produces several structurally related peptides, including glucagon-like peptide 1 (GLP-1) and GLP-2, as well as glicentin and oxyntomodulin, which contain glucagon sequence in their molecules. Among PGDPs, GLP-2 has recently been found to show intestinal epithelial proliferation.
▼ The assay kit can measure GLP-2 in the range of 0.137 – 100 ng/mL
▼ The assay completes within 16-18 hr. +1.5 hr.
▼ With one assay kit, 40 samples can be measured in duplicate
▼ Test sample: rat serum or plasma
▼ Sample volume: 25 μL
▼ The 96-well plate in kit is consisted by 8-wells strips. The kit can be used separately.
▼ Precision and reproducibility
Intra-assay CV（％） serum 3.5 – 8.9
Inter-assay CV（％） serum 7.6 – 13.0
Intra-assay CV（％） plasma 3.1 – 7.2
Inter-assay CV（％） plasma 6.7 – 11.5
< Specificity >
The EIA kit has high specificity to rat GLP-2 and shows no cross reactivity with rat glucagon and rat GLP-1 even in the concentration of 300 pmol/mL.